Objective To verify the application of anterior segment perfusion culture and trabecular meshwork ( TM) organ culture for glaucoma study. 目的了解猪眼小梁组织的灌流眼前节培养和组织块培养在青光眼研究中的应用。
The Invention of a New Corneal Perfusion Culture System and Its Application in Corneal Preservation 新型角膜灌流培养系统的研制及在角膜保存中的应用
Application of collagenase perfusion and digestion techniques in the primary culture of endothelial cells from human umbilical vein in vitro 胶原酶脐带灌注消化法在人脐带静脉内皮细胞的原代分离培养中的作用
Isolation of rat Kupffer cells by ex vivo perfusion and their primary culture 体外灌注法分离大鼠肝脏Kupffer细胞及原代培养
The design and performance test of cell perfusion culture device which could show pressure 可显压力的细胞灌注培养装置的设计及其性能测试
Perfusion Culture Method of Vero Cell on Microcarrier in Bioreactor 生物反应罐Vero细胞微载体灌流培养
To investigate the growth and the biological characteristics of MSC, common culture and perfusion culture system was adapted. 并对MSC在灌注培养条件下生物学特性进行探讨。
Fabrication of Scaffold with Controlled Porous Structure and Flow Perfusion Culture in vitro 可控多孔结构支架制备及灌注式体外培养
Using an in situ collagenase-pronase E perfusion technique, Kupffer's cells were isolated from hepatocytes in rat immunological hepatic injury, and nitric oxide ( NO) production in culture supernatant was measured by the method of the Griess's reaction. 采用胶原酶-链霉蛋白酶灌流法对免疫性肝损伤大鼠肝实质细胞与枯否细胞进行分离与原代培养,Griess反应法检测细胞培养上清中一氧化氮(NO)生成量的变化。
The data implicated that the bioreactor was available for producing taxol by continuous perfusion culture. 这些结果表明该反应器可用于连续灌注培养红豆杉细胞生产紫杉醇。
Isolation of pancreatic stellate cells using collagenase perfusion method through abdominal aorta cannulation and its culture and characterization 腹主动脉灌注法分离胰腺星状细胞及其培养、鉴定
A flow perfusion bioreactor, which allowed exposure of the culture cells to low levels of mechanical stimulation by fluid flow-induced shear stress, was developed in our lab. 构建灌注式生物反应器系统,实现氧气和营养物质的大量输送,同时产生一定流体剪应力,调节细胞功能的发挥。
Simulation of Bone Marrow Hematopoiesis Using Perfusion Culture in the Membrane of Hollow Fibers 中空纤维灌注培养模拟骨髓造血研究
Perfusion Culture of Hematopoietic Cells in a Stirred Tank Bioreactor 搅拌式生物反应器中造血细胞的灌注培养
The experimental data indicated that the perfusion culture, was excelled than that of the recirculation culture, and that the recirculation culture which had the characteristics of both perfusion and batch cultures was excelled than that of the batch culture. 表明灌流培养优于再循环培养;而再循环培养兼容批培养及灌流培养的特点,又优于批培养。
This paper has reported a method for the determination of glucose in the supernatants of microencapsulated animal cell perfusion culture using glucose biosensor. 本文提出了用葡萄糖传感器监测在灌注培养微囊化动物细胞时,培液中葡萄糖含量的变化。
Application of Glucose Biosensor to the Perfusion Culture of Microencapsulated Animal Cell 葡萄糖传感器在微囊化动物细胞灌注培养过程中的应用
Based on the formation kinetics of monoclonal antibody, the hybridoma cell culture in bioreactor was optimized by the application of perfusion culture mode, the supplementation of potassium acetate, and the fortification of nutrients. 根据杂交瘤细胞培养中单克隆抗体生产的动力学原理,采用灌注培养方式、添加醋酸钾和丰富营养物培养的途径,对反应器放大培养过程进行了优化。
A set of perfusion experiments with a paired bovine pulmonary artery endothelial cell ( BPAEC) culture monolayer was utilized to study the relationship between length of cell monolayer and its angiotensin II metabolism. 用离体培养的牛肺动脉内皮细胞灌流实验研究内皮细胞单层长度与其血管紧张素II代谢的相关性。
A New Extracorporeal Perfusion Technique for Dispersing Monkey Kidney Cell in Tissue Culture 离体肾脏灌注细胞分散技术
Methods The techniques included: ① liver perfusion in situ, ② preparation of nonparenchymal cells suspension, ③ centrifugal elutriation, ④ primary culture. 方法采用原位肝脏酶灌注、肝非实质细胞悬液的准备、离心淘洗和原代培养等方法进行分离。
Methods: Using tissue culture and sequence perfusion in hypothalamus, anterior pituitary gland and testis. The concentration of LH and T in the culture fluid was detected by radioimmunoassay ( RIA). 方法:下丘脑、腺垂体、睾丸离体组织培养以及顺序灌注,于三级组织培养液中加入不同浓度的SP,用放射免疫分析法测定培养液中LH和T的分泌量。
A vary type of packed-bed bioreactors have been used in dynamic seeding and perfusion culture of cells especially in hepatocyte high-density cultures. 目前,已经有许多种类的填充床细胞反应器被用于细胞的动态接种和灌流培养,特别是被用于肝细胞高密度培养。
To find how this new type of perfusion culture system affect the survival environ-ment of keeping hare cornea in traditional organ culture system and the activity of corneal endothelial cells, and detect the application in cornea preservation. 3. 研究此新型培养系统对器官培养保存兔角膜的生存环境、内皮细胞活性的影响,检验其在器官培养角膜保存中的应用效果。